Journal: iScience
Article Title: Pharmaceutical inhibition of the Chk2 kinase mitigates cone photoreceptor degeneration in an iPSC model of Bardet-Biedl syndrome
doi: 10.1016/j.isci.2025.112130
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Article Snippet: Primary antibodies used in this study are sheep anti-Chx10 (1:250, Exalpha Biologicals, X1180P), rabbit anti-CORD2 (CRX) (1:300, GenTex, GTX124188), rabbit anti-OTX2 (1:300, Abcam, ab183951), goat anti-OTX2 (1:500, R&D Systems, AF1979), rabbit anti-S-opsin (1:200, Invitrogen, OSO00006G), rabbit anti-M/L-opsin (1:100, Chemicon, AB5405), rabbit anti-Recoverin (1:1000, Millipore, AB5585), mouse anti-γH2AX (1:250, Millipore, 05–636), rabbit anti-53BP1 (1:250, Novus, NB100-304), rabbit anti- p -Chk2 (1:250, Cell Signaling, 2661), mouse anti- p -ATM (1:250, Millipore, MAB3806-C), rabbit anti- p -ATR (1:250, Millipore, SAB5701785), anti-Ki67 (1:500, Abcam, ab15580), rabbit anti-Histone H3 (phospho S10) (1:500, Abcam, ab5176), anti-cleaved Caspase3 (1:1000, Cell Signaling, 9661), mouse alpha acetyl tubulin (1:300, Santa Cruz, sc23950), mouse anti-AURKB (1:300, Novus, NBP2-50039), mouse anti-Centriolin (1:300, Santa Cruz, sc-365521), anti-CEP164 (1:300, Novus, NBP1-81445), PNA FITC (1:200, Vector Laboratories, FL-1071), and rhodamine phalloidin (1:200, Invitrogen, R415).
Techniques: Plasmid Preparation, Recombinant, Full Display Name, TUNEL Assay, Imaging, Ab Array, Generated, Software, Microscopy, Extraction